Characterisation of the first complete genome sequence of the roe deer (Capreolus capreolus) papillomavirus

被引:27
作者
Erdelyi, Karoly [1 ]
Balint, Adam [1 ]
Dencso, Laszlo [1 ]
Dan, Adam [1 ]
Ursu, Krisztina [1 ]
机构
[1] Vet Diagnost Directorate, Cent Agr Off, H-1149 Budapest, Hungary
关键词
papillomavirus; roe deer; tumour; fibropapilloma; genome; skin;
D O I
10.1016/j.virusres.2008.03.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The complete genomic DNA of a novel roe deer (Capreolus capreolus) papillomavirus (CcPV1) was amplified and sequenced from fibropapillomatous skin lesions of a Hungarian roe deer. Viral DNA was detected by a pair of degenerate primers and the remaining genomic sequence was amplified by a long-template high-fidelity PCR and sequenced. The CcPV1 genome was 8032 bp long and contained open reading frames (ORFs) typical for Delta-papillomaviruses (E6, E7, E1, E2, E4, E5, E9, L2, and L1) and a 799 bp long untranslated regulatory region (URR). Phylogenetic analysis based on the 3861 bp long concatenated sequence of the E1 -E2-L2-L1 ORFs and on separate alignments of all major ORFs using both neighbour-joining and maximum parsimony methods placed CcPV1 on a distinct branch between Ovine papillomavirus 1 and the other deer papillomaviruses within the Delta-papillomavirus genus, although pair-wise nucleotide alignments of L1 ORF sequences determined highest identities with European Elk Papillomavirus (71.2%) and Reindeer Papillomavirus (70.3%). (C) 2008 Elsevier B.V. All rights reserved.
引用
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页码:307 / 311
页数:5
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