Two-photon excitation profiles in cylindrical capillaries

被引：8

作者：

Overway, KS ^{[1
]}

Lytle, FE ^{[1
]}

机构：

[1] Purdue Univ, Dept Chem, Brown Lab 1393, W Lafayette, IN 47907 USA

来源：

APPLIED SPECTROSCOPY | 1998年 / 52卷 / 07期

关键词：

fluorescence; two-photon; capillary; detection volume;

D O I：

10.1366/0003702981944733

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Poor detection limits of two-photon excited fluorescence in cylindrical capillaries are attributed to photothermal expansion and beam astigmatism. Photothermal expansion is demonstrated for excitation in a 1 cm cell and is inferred for the larger diameter capillaries. Astigmatism is caused by focal differences between rays in a plane Longitudinal to the capillary and rays in a plane transverse to the capillary. Data were obtained by integrating the fluorescence from variously sized cylindrical and square capillaries, and by photographing the fluorescence within a 1 cm cell.

引用

页码：928 / 932

页数：5

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