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Highly sensitive detection using laser two-photon excited fluorescence in capillary electrophoresis

被引：9
作者：
Song, JM
Inoue, T
Kawazumi, H
Ogawa, T
机构：
[1] KYUSHU UNIV,DEPT MOL SCI & TECHNOL,KASUGA,FUKUOKA 816,JAPAN
[2] KINKI UNIV,DEPT IND CHEM,IIZUKA,FUKUOKA 820,JAPAN
来源：
JOURNAL OF CHROMATOGRAPHY A | 1997年 / 765卷 / 02期
关键词：
detection; capillary electrophoresis; two-photon laser-induced fluorescence; fluorescence detection; two-photon; 7-amino-4-methylcoumarine (coumarine 440); 7-diethylaminocoumarine 3-carboxylic acid succinimidyl ester (DCCS);
D O I：
10.1016/S0021-9673(96)00937-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The laser two-photon excited fluorescence (LTPF) has been applied to a capillary electrophoresis (CE) system using a self mode-locked titanium-sapphire laser. The analytes were coumarine 440 (7-amino-4-methyl coumarine) and DCCS (7-diethyl aminocoumarine-3-carboxylic acid succimmidyl ester). The two-photon excited fluorescence emission spectrum of DCCS was identical to the one-photon excited fluorescence emission spectrum. The LTPF quantum efficiency increased through the addition of methanol to the buffer solution, and, as a result, the detection limit decreased. The detection limit of coumarine 440 was 8.0 amol, this value was as low as that obtained by one-photon excited fluorescence.
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页码：315 / 319
页数：5
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