CAPILLARY ZONE ELECTROPHORESIS WITH TIME-RESOLVED FLUORESCENCE DETECTION USING A DIODE-PUMPED SOLID-STATE LASER

被引：23

作者：

MILLER, KJ ^{[1
]}

LYTLE, FE ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT CHEM,1393 BROWN LABS,W LAFAYETTE,IN 47907

来源：

JOURNAL OF CHROMATOGRAPHY | 1993年 / 648卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0021-9673(93)83307-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A capillary electrophoresis system with time-resolved fluorescence detection was developed using a diode-pumped solid-state laser. The laser provides 2.5-ns pulses at 349 nm with kHz repetition rates, and the Gaussian-shaped beam can easily be focused to the dimensions of a capillary. The short pulse width makes it possible to achieve nanosecond resolution and time-filtered detection. With this approach, the total signal from one injection can be collected and digitally processed to yield simultaneously ''two-channel'' data. Instrument capability is demonstrated by resolving short-lived fluorescent interferences in biological sample matrixes from long-lived fluorophores.

引用

页码：245 / 250

页数：6

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