A high-resolution radiation hybrid map of the human genome draft sequence

被引：89

作者：

Olivier, M

Aggarwal, A

Allen, J

Almendras, AA

Bajorek, ES

Beasley, EM

Brady, SD

Bushard, JM

Bustos, VI

Chu, A

Chung, TR

De Witte, A

Denys, ME

Dominguez, R

Fang, NY

Foster, BD

Freudenberg, RW

Hadley, D

Hamilton, LR

Jeffrey, TJ

Kelly, L

Lazzeroni, L

Levy, MR

Lewis, SC

Liu, X

Lopez, FJ

Louie, B

Marquis, JP

Martinez, RA

Matsuura, MK

Misherghi, NS

Norton, JA

Olshen, A

Perkins, SM

Perou, AJ

Piercy, C

Piercy, M

Qin, F

Reif, T

Sheppard, K

Shokoohi, V

Smick, GA

Sun, WL

Stewart, EA

Tejeda, JF

Tran, NM

Trejo, T

Vo, NT

Yan, SCM

Zierten, DL

机构：

[1] Stanford Univ, Stanford Human Genome Ctr, Sch Med, Palo Alto, CA 94304 USA

[2] Inst Genome Res, Rockville, MD 20850 USA

[3] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[4] Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA

来源：

SCIENCE | 2001年 / 291卷 / 5507期

关键词：

D O I：

10.1126/science.1057437

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNC radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

引用

页码：1298 / +

页数：6

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