Functional analysis of sigH expression in Coryhebacterium glutamicum

被引：43

作者：

Kim, TH

Kim, HJ

Park, JS

Kim, Y

Kim, P

Lee, HS ^{[1
]}

机构：

[1] Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea

[2] Semyung Univ, Dept Oriental Med, Checheon 390230, Chungbuk, South Korea

[3] Catholic Univ Korea, Div Biotechnol, Bucheum 420743, Kyungki, South Korea

[4] Korea Univ, Dept Biotechnol & Bioinformat, Jochiwon 339700, Chungnam, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 331卷 / 04期

关键词：

Corynebacterium glutamicum; sigH; heat stress; oxidative stress; trxB; diamide;

D O I：

10.1016/j.bbrc.2005.04.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sigH gene of Corynebacterium glutomicum encodes ECF sigma factor sigma(H). The gene apparently plays an important role in other stress responses as well as heat stress response. In this study, we found that deleting the sigH gene made C. glutamicum cells sensitive to the thiol-specific oxidant diamide. In the sigH mutant strain, the activity of thioredoxin reductase markedly decreased, suggesting that the trxB gene encoding thioredoxin reductase is probably under the control of sigma(H). The expression of sigH was stimulated in the stationary growth phase and modulated by diamide. In addition, the SigH protein was required for the expression of its own gene. These data indicate that the sigH gene of C. glutamicum stimulates and regulates its own expression in the stationary growth phase in response to environmental stimuli, and participates in the expression of other genes which are important for survival following heat and oxidative stress response. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：1542 / 1547

页数：6

共 31 条

[1] Redox-dependent changes in RsrA, an anti-sigma factor in Streptomyces coelicolor:: Zinc release and disulfide bond formation [J].

Bae, JB ;

Park, JH ;

Hahn, MY ;

Kim, MS ;

Roe, JH .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 335 (02) :425-435

[2] Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) [J].

Bentley, SD ;

Chater, KF ;

Cerdeño-Tárraga, AM ;

Challis, GL ;

Thomson, NR ;

James, KD ;

Harris, DE ;

Quail, MA ;

Kieser, H ;

Harper, D ;

Bateman, A ;

Brown, S ;

Chandra, G ;

Chen, CW ;

Collins, M ;

Cronin, A ;

Fraser, A ;

Goble, A ;

Hidalgo, J ;

Hornsby, T ;

Howarth, S ;

Huang, CH ;

Kieser, T ;

Larke, L ;

Murphy, L ;

Oliver, K ;

O'Neil, S ;

Rabbinowitsch, E ;

Rajandream, MA ;

Rutherford, K ;

Rutter, S ;

Seeger, K ;

Saunders, D ;

Sharp, S ;

Squares, R ;

Squares, S ;

Taylor, K ;

Warren, T ;

Wietzorrek, A ;

Woodward, J ;

Barrell, BG ;

Parkhill, J ;

Hopwood, DA .

NATURE, 2002, 417 (6885) :141-147

[3] Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stress [J].

Carmel-Harel, O ;

Storz, G .

ANNUAL REVIEW OF MICROBIOLOGY, 2000, 54 :439-461

[4] Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].

Cole, ST ;

Brosch, R ;

Parkhill, J ;

Garnier, T ;

Churcher, C ;

Harris, D ;

Gordon, SV ;

Eiglmeier, K ;

Gas, S ;

Barry, CE ;

Tekaia, F ;

Badcock, K ;

Basham, D ;

Brown, D ;

Chillingworth, T ;

Connor, R ;

Davies, R ;

Devlin, K ;

Feltwell, T ;

Gentles, S ;

Hamlin, N ;

Holroyd, S ;

Hornby, T ;

Jagels, K ;

Krogh, A ;

McLean, J ;

Moule, S ;

Murphy, L ;

Oliver, K ;

Osborne, J ;

Quail, MA ;

Rajandream, MA ;

Rogers, J ;

Rutter, S ;

Seeger, K ;

Skelton, J ;

Squares, R ;

Squares, S ;

Sulston, JE ;

Taylor, K ;

Whitehead, S ;

Barrell, BG .

NATURE, 1998, 393 (6685) :537-+

[5] clpC and clpP1P2 gene expression in Corynebacterium glutamicum is controlled by a regulatory network involving the transcriptional regulators ClgR and HspR as well as the ECF sigma factor σH [J].

Engels, S ;

Schweitzer, JE ;

Ludwig, C ;

Bott, M ;

Schaffer, S .

MOLECULAR MICROBIOLOGY, 2004, 52 (01) :285-302

[6] GENE STRUCTURE AND EXPRESSION OF THE CORYNEBACTERIUM-FLAVUM N-13 ASK-ASD OPERON [J].

FOLLETTIE, MT ;

PEOPLES, OP ;

AGOROPOULOU, C ;

SINSKEY, AJ .

JOURNAL OF BACTERIOLOGY, 1993, 175 (13) :4096-4103

[7] INTRACELLULAR PRODUCTION OF SUPEROXIDE RADICAL AND OF HYDROGEN-PEROXIDE BY REDOX ACTIVE COMPOUNDS [J].

HASSAN, HM ;

FRIDOVICH, I .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1979, 196 (02) :385-395

[8]

Helmann JD, 2002, ADV MICROB PHYSIOL, V46, P47

[9]

HOLMGREN A, 1985, ANNU REV BIOCHEM, V54, P237, DOI 10.1146/annurev.biochem.54.1.237

[10]

HOLMGREN A, 1979, J BIOL CHEM, V254, P9113

← 1 2 3 4 →