Crystal structure of the mosquito-iarvicidal toxin Cry4Ba and its biological implications

被引:175
作者
Boonserm, P
Davis, P
Ellar, DJ
Li, J
机构
[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England
基金
英国医学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
bioinsecticide; proteolytic activation; target specificity; toxin-membrane interaction; hydrophobic patch;
D O I
10.1016/j.jmb.2005.02.013
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cry4Ba, isolated from Bacillus thuringiensis subsp. israelensis, is specifically toxic to the larvae of Aedes and Anopheles mosquitoes. The structure of activated Cry4Ba toxin has been determined by multiple isomorphous replacement with anomalous scattering and refined to R-cryst=20.5% and R-free=21.8% at 1.75 A resolution. It resembles previously reported Cry toxin structures but shows the following distinctions. In domain I the helix bundle contains only the long and amphipathic helices alpha 3-alpha 7. The N-terminal helices alpha 1-alpha 2b, absent due to proteolysis during crystallisation, appear inessential to toxicity. In domain 11 the beta-sheet prism presents short apical loops without the beta-ribbon extension of inner strands, thus placing the receptor combining sites close to the sheets. In domain III the beta-sandwich contains a helical extension from the C-terminal strand 23, which interacts with a beta-hairpin excursion from the edge of the outer sheet. The structure provides a rational explanation of recent mutagenesis and biophysical data on this toxin. Furthermore, added to earlier structures from the Cry toxin family, Cry4Ba completes a minimal structural database covering the Coleoptera, Lepidoptera, Diptera and Lepidoptera/Diptera specificity classes. A multiple structure alignment found that the Diptera-specific Cry4Ba is structurally more closely similar to the Lepidoptera-specific Cry1Aa than the Coleoptera-specific Cry3Aa, but most distantly related to Lepicloptera/Diptera-specific Cry2Aa. The structures are most divergent in domain 11, supporting the suggestion that this domain has a major role in specificity determination. They are most similar in the alpha 3-alpha 7 major fragment of domain 1, which contains the alpha 4-alpha 5 hairpin crucial to pore formation. The collective knowledge of Cry toxin structure and mutagenesis data will lead to a more critical understanding of the structural basis for receptor binding and pore formation, as well as allowing the scope of diversity to be better appreciated. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:363 / 382
页数:20
相关论文
共 80 条
[71]  
Sramala I., 2001, J Biochem Mol Biol Biophys, V5, P219
[72]  
Uawithya P, 1998, BIOCHEM MOL BIOL INT, V44, P825
[73]   Role of helix 3 in pore formation by the Bacillus thuringiensis insecticidal toxin Cry1Aa [J].
Vachon, V ;
Préfontaine, G ;
Coux, F ;
Rang, C ;
Marceau, L ;
Masson, M ;
Brousseau, R ;
Frutos, R ;
Schwartz, JL ;
Laprade, R .
BIOCHEMISTRY, 2002, 41 (19) :6178-6184
[74]  
VADLAMUDI RK, 1993, J BIOL CHEM, V268, P12334
[75]   CLONING AND EXPRESSION OF A RECEPTOR FOR AN INSECTICIDAL TOXIN OF BACILLUS-THURINGIENSIS [J].
VADLAMUDI, RK ;
WEBER, E ;
JI, IH ;
JI, TH ;
BULLA, LA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (10) :5490-5494
[76]   LOCATION OF THE DIPTERAN SPECIFICITY REGION IN A LEPIDOPTERAN-DIPTERAN CRYSTAL PROTEIN FROM BACILLUS-THURINGIENSIS [J].
WIDNER, WR ;
WHITELEY, HR .
JOURNAL OF BACTERIOLOGY, 1990, 172 (06) :2826-2832
[77]   Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles [J].
Wolfersberger, MG ;
Chen, XJ ;
Dean, DH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (01) :279-282
[78]  
WU D, 1992, J BIOL CHEM, V267, P2311
[79]   Functional significance of loops in the receptor binding domain of Bacillus thuringiensis CryIIIA delta-endotoxin [J].
Wu, SJ ;
Dean, DH .
JOURNAL OF MOLECULAR BIOLOGY, 1996, 255 (04) :628-640
[80]   ISOLATION OF A PROTEIN FROM THE PARASPORAL CRYSTAL OF BACILLUS-THURINGIENSIS VAR KURSTAKI TOXIC TO THE MOSQUITO LARVA, AEDES-TAENIORHYNCHUS [J].
YAMAMOTO, T ;
MCLAUGHLIN, RE .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 103 (02) :414-421