One-step metal-affinity method for the purification of the iron-binding protein Fbp of Neisseria meningitidis

被引:4
作者
Ferron, L [1 ]
Pintor, M [1 ]
Gomez, JA [1 ]
Criado, MT [1 ]
Ferreiros, C [1 ]
机构
[1] UNIV SANTIAGO DE COMPOSTELA,FAC FARM,DEPT MICROBIOL & PARASITOL,SANTIAGO COMPOSTE,SPAIN
关键词
metal-affinity method; iron-binding protein (Fbp); Neisseria meningitidis;
D O I
10.1016/0167-7012(95)00091-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A one-step method for the purification of the iron-binding protein, Fbp, from Neisseria meningitidis has been developed using affinity chromatography on iron-Sepharose. This method allowed the purification of the protein in non-denaturing conditions and in a similar way to those used previously. Our results demonstrate the homogeneity of the purified protein as tested by SDS-PAGE and IEF.
引用
收藏
页码:233 / 236
页数:4
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