A small molecule-kinase interaction map for clinical kinase inhibitors

被引:1588
作者
Fabian, MA [1 ]
Biggs, WH [1 ]
Treiber, DK [1 ]
Atteridge, CE [1 ]
Azimioara, MD [1 ]
Benedetti, MG [1 ]
Carter, TA [1 ]
Ciceri, P [1 ]
Edeen, PT [1 ]
Floyd, M [1 ]
Ford, JM [1 ]
Galvin, M [1 ]
Gerlach, JL [1 ]
Grotzfeld, RM [1 ]
Herrgard, S [1 ]
Insko, DE [1 ]
Insko, MA [1 ]
Lai, AG [1 ]
Lélias, JM [1 ]
Mehta, SA [1 ]
Milanov, ZV [1 ]
Velasco, AM [1 ]
Wodicka, LM [1 ]
Patel, HK [1 ]
Zarrinkar, PP [1 ]
Lockhart, DJ [1 ]
机构
[1] Ambit Biosci, San Diego, CA 92121 USA
关键词
D O I
10.1038/nbt1068
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Kinase inhibitors show great promise as a new class of therapeutics. Here we describe an efficient way to determine kinase inhibitor specificity by measuring binding of small molecules to the ATP site of kinases. We have profiled 20 kinase inhibitors, including 16 that are approved drugs or in clinical development, against a panel of 119 protein kinases. We find that specificity varies widely and is not strongly correlated with chemical structure or the identity of the intended target. Many novel interactions were identified, including tight binding of the p38 inhibitor BIRB-796 to an imatinib-resistant variant of the ABL kinase, and binding of imatinib to the SRC-family kinase LICK. We also show that mutations in the epidermal growth factor receptor (EGFR) found in gefitinib-responsive patients do not affect the binding affinity of gefitinib or erlotinib. Our results represent a systematic small molecule-protein interaction map for clinical compounds across a large number of related proteins.
引用
收藏
页码:329 / 336
页数:8
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