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Metabolomics technologies and metabolite identification

被引：280
作者：
Moco, Sofia
Bino, Raoul J.
De Vos, Ric C. H.
Vervoort, Jacques
机构：
[1] Univ Wageningen & Res Ctr, Biochem Lab, NL-6703 HA Wageningen, Netherlands
[2] Ctr BioSyst Genom, NL-6700 AB Wageningen, Netherlands
[3] Plant Res Int, NL-6700 AA Wageningen, Netherlands
[4] Univ Wageningen & Res Ctr, Lab Plant Physiol, NL-6703 BD Wageningen, Netherlands
来源：
TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2007年 / 26卷 / 09期
关键词：
Bioinformatics; database; identification; liquid chromatography; mass spectrometry; metabolite; metabolome; metabolomics; metabonomics; nuclear magnetic resonance;
D O I：
10.1016/j.trac.2007.08.003
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Metabolomics studies rely on the analysis of the multitude of small molecules (metabolites) present in a biological system. Most commonly, metabolomics is heavily supported by mass spectrometry (MS) and nuclear magnetic resonance (NMR) as parallel technologies that provide an overview of the metabolome and high-power compound elucidation. Over and above large-scale analysis, a major effort is needed for unequivocal identification of metabolites. The combination of liquid chromatography (LC)-MS and NMR is a powerful methodology for identifying metabolites. Better chemical characterization of the metabolome will undoubtedly enlarge knowledge of any biological system. (c) 2007 Elsevier Ltd. All rights reserved.
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页码：855 / 866
页数：12
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