Metabolomics technologies and metabolite identification

被引：280

作者：

Moco, Sofia

Bino, Raoul J.

De Vos, Ric C. H.

Vervoort, Jacques

机构：

[1] Univ Wageningen & Res Ctr, Biochem Lab, NL-6703 HA Wageningen, Netherlands

[2] Ctr BioSyst Genom, NL-6700 AB Wageningen, Netherlands

[3] Plant Res Int, NL-6700 AA Wageningen, Netherlands

[4] Univ Wageningen & Res Ctr, Lab Plant Physiol, NL-6703 BD Wageningen, Netherlands

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2007年 / 26卷 / 09期

关键词：

Bioinformatics; database; identification; liquid chromatography; mass spectrometry; metabolite; metabolome; metabolomics; metabonomics; nuclear magnetic resonance;

D O I：

10.1016/j.trac.2007.08.003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Metabolomics studies rely on the analysis of the multitude of small molecules (metabolites) present in a biological system. Most commonly, metabolomics is heavily supported by mass spectrometry (MS) and nuclear magnetic resonance (NMR) as parallel technologies that provide an overview of the metabolome and high-power compound elucidation. Over and above large-scale analysis, a major effort is needed for unequivocal identification of metabolites. The combination of liquid chromatography (LC)-MS and NMR is a powerful methodology for identifying metabolites. Better chemical characterization of the metabolome will undoubtedly enlarge knowledge of any biological system. (c) 2007 Elsevier Ltd. All rights reserved.

引用

收藏

页码：855 / 866

页数：12

相关论文

共 49 条

[21] Metabolite fingerprinting and profiling in plants using NMR [J].

Krishnan, P ;

Kruger, NJ ;

Ratcliffe, RG .

JOURNAL OF EXPERIMENTAL BOTANY, 2005, 56 (410) :255-265

[22] Metabolite profiling of tomato (Lycopersicon esculentum) using 1H NMR spectroscopy as a tool to detect potential unintended effects following a genetic modification [J].

Le Gall, G ;

Colquhoun, IJ ;

Davis, AL ;

Collins, GJ ;

Verhoeyen, ME .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (09) :2447-2456

[23] Gas chromatography mass spectrometry-based metabolite profiling in plants [J].

Lisec, Jan ;

Schauer, Nicolas ;

Kopka, Joachim ;

Willmitzer, Lothar ;

Fernie, Alisdair R. .

NATURE PROTOCOLS, 2006, 1 (01) :387-396

[24] Performance evaluation of a hybrid linear ion trap/orbitrap mass spectrometer [J].

Makarov, A ;

Denisov, E ;

Kholomeev, A ;

Baischun, W ;

Lange, O ;

Strupat, K ;

Horning, S .

ANALYTICAL CHEMISTRY, 2006, 78 (07) :2113-2120

[25] A study of ion suppression effects in electrospray ionization from mobile phase additives and solid-phase extracts [J].

Mallet, CR ;

Lu, ZL ;

Mazzeo, JR .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (01) :49-58

[26] MIRACLE:: mass isotopomer ratio analysis of U-13C-labeled extracts.: A new method for accurate quantification of changes in concentrations of intracellular metabolites [J].

Mashego, MR ;

Wu, L ;

Van Dam, JC ;

Ras, C ;

Vinke, JL ;

Van Winden, WA ;

Van Gulik, WM ;

Heijnen, JJ .

BIOTECHNOLOGY AND BIOENGINEERING, 2004, 85 (06) :620-628

[27] Discrimination of wines based on 2D NMR spectra using learning vector quantization neural networks and partial least squares discriminant analysis [J].

Masoum, S ;

Bouveresse, DJR ;

Vercauteren, J ;

Jalali-Heravi, M ;

Rutledge, DN .

ANALYTICA CHIMICA ACTA, 2006, 558 (1-2) :144-149

[28] Mass analysis at the advent of the 21st century [J].

McLuckey, SA ;

Wells, JM .

CHEMICAL REVIEWS, 2001, 101 (02) :571-606

[29]

MOCO S, 2006, J VERVOORT CHROMATOG, V9, P503

[30] A liquid chromatography-mass spectrometry-based metabolome database for tomato [J].

Moco, Sofia ;

Bino, Raoul J. ;

Vorst, Oscar ;

Verhoeven, Harrie A. ;

de Groot, Joost ;

van Beek, Teris A. ;

Vervoort, Jacques ;

de Vos, C. H. Ric .

PLANT PHYSIOLOGY, 2006, 141 (04) :1205-1218

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