Discovery of a novel protein modification: alpha-glycerophosphate is a substituent of meningococcal pilin

被引:69
作者
Stimson, E
Virji, M
Barker, S
Panico, M
Blench, I
Saunders, J
Payne, G
Moxon, ER
Dell, A
Morris, HR
机构
[1] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AY,ENGLAND
[2] UNIV OXFORD,JOHN RADCLIFFE HOSP,DEPT PAEDIAT,OXFORD OX3 9DU,ENGLAND
[3] UNIV LIVERPOOL,DEPT GENET & MICROBIOL,LIVERPOOL L69 3BX,MERSEYSIDE,ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1042/bj3160029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pili, which are filamentous protein structures on the surface of the meningitis-causing organism Neisseria meningitidis, are known to be post-translationally modified with substituents that affect their mobility in SDS/PAGE and which might play a crucial role in adherence and bloodstream invasion. Tryptic digests of pili were analysed by fast atom bombardment and electrospray MS to identify putative modifications. Serine-93 was found to carry a novel modification of alpha-glycerophosphate. This is the first time that alpha-glycerophosphate has been observed as a substituent of a prokaryotic or eukaryotic protein.
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收藏
页码:29 / 33
页数:5
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