Transverse fluctuation analysis of single extended DNA molecules

被引：6

作者：

Gueroui, Z ^{[1
]}

Freyssingeas, E ^{[1
]}

Place, C ^{[1
]}

Berge, B ^{[1
]}

机构：

[1] Ecole Normale Super Lyon, Phys Lab, CNRS, UMR 5672, F-69007 Lyon, France

来源：

EUROPEAN PHYSICAL JOURNAL E | 2003年 / 11卷 / 01期

关键词：

D O I：

10.1140/epje/i2002-10150-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We observed fluctuations of elongated DNA molecules by fluorescence microscopy. The molecules are fixed at both ends and undulate. Mode analysis of the thermally excited undulations of the labeled DNA molecules gives access to the spectral density of the amplitude fluctuations. From these measurements we estimate the tension acting on the DNA molecules. We found the forces to be within the entropic elasticity range of a typical DNA molecule (measured on the single-molecule level).

引用

页码：105 / 108

页数：4

共 9 条

[1] ENTROPIC ELASTICITY OF LAMBDA-PHAGE DNA [J].

BUSTAMANTE, C ;

MARKO, JF ;

SIGGIA, ED ;

SMITH, S .

SCIENCE, 1994, 265 (5178) :1599-1600

[2] Dynamics of a tethered polymer in shear flow [J].

Doyle, PS ;

Ladoux, B ;

Viovy, JL .

PHYSICAL REVIEW LETTERS, 2000, 84 (20) :4769-4772

[3]

GUEROUI Z, UNPUB LANGMUIR

[4] DIRECT MEASUREMENT OF THE WAVE-VECTOR-DEPENDENT BENDING STIFFNESS OF FREELY FLICKERING ACTIN-FILAMENTS [J].

KAS, J ;

STREY, H ;

BARMANN, M ;

SACKMANN, E .

EUROPHYSICS LETTERS, 1993, 21 (08) :865-870

[5] Dynamics of individual flexible polymers in a shear flow [J].

LeDuc, P ;

Haber, C ;

Bao, G ;

Wirtz, D .

NATURE, 1999, 399 (6736) :564-566

[6] Single polymer dynamics in an elongational flow [J].

Perkins, TT ;

Smith, DE ;

Chu, S .

SCIENCE, 1997, 276 (5321) :2016-2021

[7] RELAXATION OF A SINGLE DNA MOLECULE OBSERVED BY OPTICAL MICROSCOPY [J].

PERKINS, TT ;

QUAKE, SR ;

SMITH, DE ;

CHU, S .

SCIENCE, 1994, 264 (5160) :822-826

[8] The dynamics of partially extended single molecules of DNA [J].

Quake, SR ;

Babcock, H ;

Chu, S .

NATURE, 1997, 388 (6638) :151-154

[9] DIRECT MECHANICAL MEASUREMENTS OF THE ELASTICITY OF SINGLE DNA-MOLECULES BY USING MAGNETIC BEADS [J].

SMITH, SB ;

FINZI, L ;

BUSTAMANTE, C .

SCIENCE, 1992, 258 (5085) :1122-1126

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