Multilayer membranes via layer-by-layer deposition of PDDA and DNA with Au nanoparticles as tags for DNA biosensing

被引：26

作者：

Chang, Zhu ^{[1
,2
]}

Chen, Miao ^{[1
]}

Fan, Hao ^{[1
]}

Zhao, Kun ^{[1
]}

Zhuang, Shuqi ^{[1
]}

He, Pingang ^{[1
]}

Fang, Yuzhi ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

[2] Shangqiu Teachers Coll, Dept Chem, Shanghai 476000, Peoples R China

来源：

ELECTROCHIMICA ACTA | 2008年 / 53卷 / 06期

基金：

中国国家自然科学基金;

关键词：

LBL; Au nanoparticles; PDDA; DNA biosensor; electrochemical detection;

D O I：

10.1016/j.electacta.2007.11.004

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A novel An nanoparticles (Au-NPs)-based protocol for DNA hybridization detection based on assembly of alternating DNA and poly (dimethyldiallylammonium chloride) (PDDA) multilayer films by layer-by-layer (LBL) electrostatic adsorption has been studied. Electrochemical impedance spectroscopy (EIS) and UV-vis absorbance measurements were used to study the film assembly. All the results indicate that the uniform multilayer can be obtained on the polypyrrole (PPy) coated electrode surface and the hybridization reaction can be amplified by the layer-by-layer progress. The hybridization was detected by the reductive signal of Au-NPs and nonspecific adsorption was greatly. eliminated by an unrelated DNA sequence to the tat-get DNA. Under optimum conditions, a significant sensitivity enhancement had been obtained, and the detection limit was down to 3.20 x 10(-14) M when 6 layers assembled. The DNA biosensor has good stability and reproducibility. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：2939 / 2945

页数：7

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