Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient

被引：78

作者：

Görg, A ^{[1
]}

Boguth, G ^{[1
]}

Obermaier, C ^{[1
]}

Weiss, W ^{[1
]}

机构：

[1] Tech Univ Munchen, Lehrstuhl Allgemeine Lebensmitteltechnol, Dept Food Technol, D-85350 Freising, Germany

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 8-9期

基金：

美国国家科学基金会;

关键词：

alkaline proteins; wide immobilized pH gradient; mouse liver; ribosomal proteins; two-dimensional polyacrylamide gel electrophoresis;

D O I：

10.1002/elps.1150190850

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

For checking theoretical two-dimensional (2-D) maps derived from sequenced genomes, indicating that nonnegligible amounts of proteins up to pH 12 are to be expected, a wide-range immobilized pH 4-12 gradient was generated. Depending on the extraction method of sample preparation, proteins with pls up to pH 12 are detected in a single gel. Highly reproducible protein patterns focused to the steady state with round-shaped spots up to pH 12 are obtained with the standard protocol originally described in 1988 (Gorg et al., Electrophoresis 1988, 9, 531-546).

引用

页码：1516 / 1519

页数：4

共 18 条

[11] IMPROVED SILVER STAINING PROCEDURE FOR FAST STAINING IN PHASTSYSTEM DEVELOPMENT UNIT .1. STAINING OF SODIUM DODECYL-SULFATE GELS
HEUKESHOVEN, J
DERNICK, R
[J]. ELECTROPHORESIS, 1988, 9 (01) : 28 - 32
[12] Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae
Himmelreich, R
Hilbert, H
Plagens, H
Pirkl, E
Li, BC
Herrmann, R
[J]. NUCLEIC ACIDS RESEARCH, 1996, 24 (22) : 4420 - 4449
[13] Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12
Link, AJ
Robison, K
Church, GM
[J]. ELECTROPHORESIS, 1997, 18 (08) : 1259 - 1313
[14] OFARRELL PH, 1975, J BIOL CHEM, V250, P4007
[15] HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF BASIC AS WELL AS ACIDIC PROTEINS
OFARRELL, PZ
GOODMAN, HM
OFARRELL, PH
[J]. CELL, 1977, 12 (04) : 1133 - 1141
[16] SAMPLE APPLICATION BY IN-GEL REHYDRATION IMPROVES THE RESOLUTION OF 2-DIMENSIONAL ELECTROPHORESIS WITH IMMOBILIZED PH GRADIENTS IN THE FIRST-DIMENSION
RABILLOUD, T
VALETTE, C
LAWRENCE, JJ
[J]. ELECTROPHORESIS, 1994, 15 (12) : 1552 - 1558
[17] Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients
Rabilloud, T
Adessi, C
Giraudel, A
Lunardi, J
[J]. ELECTROPHORESIS, 1997, 18 (3-4) : 307 - 316
[18] Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients
Sanchez, JC
Rouge, V
Pisteur, M
Ravier, F
Tonella, L
Moosmayer, M
Wilkins, MR
Hochstrasser, DF
[J]. ELECTROPHORESIS, 1997, 18 (3-4) : 324 - 327

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