Study of cytokinin separation using capillary electrophoresis with cyclodextrin additives

The separation of a set of 10 cytokinins by capillary electrophoresis was investigated. The influence of various cyclodextrin derivatives (alpha-, beta-, gamma-, hydroxypropyl-beta- and dimethyl-beta-cyclodextrins) in the background electrolyte on the migration characteristics of the compounds investigated was studied. The relationship between the structures of single cytokinins and the type of cyclodextrin used was evaluated. A set of all 10 cytokinins was successfully separated in 100 mmol l(-1) phosphate-Tris buffer (pH 2.5) as a background electrolyte under different experimental conditions e.g., with addition of 25 mmol l(-1) gamma-cyclodextrin. (C) 1998 Elsevier Science B.V. All rights reserved.