Single molecule measurements within individual membrane-bound ion channels using a polymer-based bilayer lipid membrane chip

被引：40

作者：

Hromada, Louis P. ^{[1
]}

Nablo, Brian J. ^{[2
]}

Kasianowicz, John J. ^{[2
]}

Gaitan, Michael A. ^{[2
]}

DeVoe, Don L. ^{[1
]}

机构：

[1] Univ Maryland, Dept Mech Engn, College Pk, MD 20742 USA

[2] NIST, Elect & Elect Engn Lab, Gaithersburg, MD 20899 USA

来源：

LAB ON A CHIP | 2008年 / 8卷 / 04期

关键词：

D O I：

10.1039/b716388f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented.

引用

页码：602 / 608

页数：7

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