Ambineela, an unusual blue protein isolated from the archaeon Acidianus ambivalens

被引：2

作者：

Gomes, CM ^{[1
]}

Teixeira, M ^{[1
]}

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 249卷 / 01期

关键词：

archaea; thermophile; ADP; flavin;

D O I：

10.1006/bbrc.1998.9020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel blue protein, named ambineela, was isolated from the soluble extract of the thermoacidophilic archaeon Acidianus ambivalens. In solution, the purified protein is a monomer with 50 kDa and has a basic character (pI similar to 8.7). The electronic spectrum shows two bands, centred at 395 and 625 nm (A(625)/A(395) = 0.7). The protein does not contain any transition metal; its blue colour is due to an unidentified non-fluorescent cofactor, covalently bound to it. Ambineela N-terminal sequence exhibits a consensus ADP-binding region, suggesting that its unknown cofactor may comprise this molecule or an analogue. (C) 1998 Academic Press.

引用

页码：23 / 25

页数：3

共 19 条

[1] ENZYMES AND PROTEINS FROM ORGANISMS THAT GROW NEAR AND ABOVE 100-DEGREES-C [J].

ADAMS, MWW .

ANNUAL REVIEW OF MICROBIOLOGY, 1993, 47 :627-658

[2]

ANEMULLER S, 1994, FEMS MICROBIOL LETT, V117, P275, DOI 10.1111/j.1574-6968.1994.tb06779.x

[3] Paramagnetic NMR analysis of the seven-iron ferredoxin from the hyperthermoacidophilic archaeon Desulfurolobus ambivalens reveals structural similarity to other dicluster ferredoxins [J].

Bentrop, D ;

Bertini, I ;

Luchinat, C ;

Mendes, J ;

Piccioli, M ;

Teixeira, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 236 (01) :92-99

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].

Bult, CJ ;

White, O ;

Olsen, GJ ;

Zhou, LX ;

Fleischmann, RD ;

Sutton, GG ;

Blake, JA ;

FitzGerald, LM ;

Clayton, RA ;

Gocayne, JD ;

Kerlavage, AR ;

Dougherty, BA ;

Tomb, JF ;

Adams, MD ;

Reich, CI ;

Overbeek, R ;

Kirkness, EF ;

Weinstock, KG ;

Merrick, JM ;

Glodek, A ;

Scott, JL ;

Geoghagen, NSM ;

Weidman, JF ;

Fuhrmann, JL ;

Nguyen, D ;

Utterback, TR ;

Kelley, JM ;

Peterson, JD ;

Sadow, PW ;

Hanna, MC ;

Cotton, MD ;

Roberts, KM ;

Hurst, MA ;

Kaine, BP ;

Borodovsky, M ;

Klenk, HP ;

Fraser, CM ;

Smith, HO ;

Woese, CR ;

Venter, JC .

SCIENCE, 1996, 273 (5278) :1058-1073

[6]

DANIELS L, 1993, BIOCH ARCHAEA

[7] Knowledge-based protein secondary structure assignment [J].

Frishman, D ;

Argos, P .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1995, 23 (04) :566-579

[8] 16S rDNA-based phylogeny of the archaeal order Sulfolobales and reclassification of Desulfurolobus ambivalens as Acidianus ambivalens comb nov [J].

Fuchs, T ;

Huber, H ;

Burggraf, S ;

Stetter, KO .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (01) :56-60

[9] Functional properties of the quinol oxidase from Acidianus ambivalens and the possible catalytic role of its electron donor - Studies on the membrane-integrated and purified enzyme [J].

Giuffre, A ;

Gomes, CM ;

Antonini, G ;

DItri, E ;

Teixeira, M ;

Brunori, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 250 (02) :383-388

[10] The NADH oxidase from the thermoacidophilic archaea Acidianus ambivalens:: Isolation and physicochemical characterisation [J].

Gomes, CM ;

Teixeira, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 243 (02) :412-415

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