Extraction and detection of DNA from Bacillus anthracis spores and the vegetative cells within 1 min

被引：63

作者：

Aslan, Kadir ^{[1
,2
]}

Previte, Michael J. R. ^{[1
,2
]}

Zhang, Yongxia ^{[1
,2
]}

Gallagher, Theresa ^{[3
]}

Baillie, Les ^{[4
]}

Geddes, Chris D. ^{[1
,2
]}

机构：

[1] Univ Maryland, Inst Biotechnol, Inst Fluorescence, Lab Adv Med Plasmon, Baltimore, MD 21201 USA

[2] Univ Maryland, Inst Biotechnol, Ctr Med Biotechnol, Lab Adv Fluorescence Spect, Baltimore, MD 21201 USA

[3] Univ Maryland, Inst Biotechnol, Ctr Med Biotechnol, Biodef Initiat, Baltimore, MD 21201 USA

[4] Cardiff Univ, Welsh Sch Pharm, Cardiff CF10 3NB, Wales

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 11期

关键词：

D O I：

10.1021/ac800519r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of a combination of low-cost technologies to both extract and detect anthrax DNA from spores and vegetative cells in two steps within 1 min is described. In a cavity, microwave energy is highly focused using thin-film aluminum "bow-tie" structures, to extract DNA from whole spores within 20 s. The detection of the released DNA, from less than 1000 vegetative cells, without additional preprocessing steps is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. The new platform technology presented here is a highly attractive alternative method for DNA extraction and the fast detection of Gram-positive bacteria and potentially other pathogenic species and cells as well.

引用

页码：4125 / 4132

页数：8

共 27 条

[1] Requirements for the development of Bacillus anthracis spore reference materials used to test detection systems [J].