Cloning and characterization of the gene coding for the aerobic azoreductase from Pigmentiphaga kullae K24

被引：67

作者：

Blümel, S ^{[1
]}

Stolz, A ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Mikrobiol, D-70569 Stuttgart, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2003年 / 62卷 / 2-3期

关键词：

D O I：

10.1007/s00253-003-1316-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene coding for an aerobic azoreductase was cloned from Pigmentiphaga kullae K24, which is able to grow with the carboxylated azo compound 1-(4'-carboxyphenylazo)-4-naphthol (carboxy-Orange 1) as sole source of carbon and energy. The gene encoded a protein with a molecular weight of 20,557 Da, with a conserved putative NAD(P)H-binding site in the amino-terminal region. The deduced amino acid sequence showed no further significant sequence homologies to previously studied aerobic azoreductases. The azoreductase was heterologously expressed in Escherichia coli and shown to convert the sulfonated azo dye Orange I and furthermore Magneson II [4-(4-nitrophenylazo)-1-naphthol].

引用

页码：186 / 190

页数：5

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