The cell cycle of Entamoeba invadens during vegetative growth and differentiation

被引：14

作者：

Ganguly, A ^{[1
]}

Lohia, A ^{[1
]}

机构：

[1] Bose Inst, Dept Biochem, Kolkata 700054, W Bengal, India

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 2001年 / 112卷 / 02期

关键词：

Entamoeba; cell cycle; DNA synthesis; encystation; excystation; vegetative growth; intracellular calcium;

D O I：

10.1016/S0166-6851(00)00376-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cell division cycle of Entamoeba invadens was studied during vegetative growth of trophozoites and during their differentiation into cysts. During vegetative growth of trophozoites, it was observed that DNA synthesis typically continued after one genome content had been duplicated. During encystation, DNA synthesis was arrested after 4n genome content had been synthesised. Using multi-parameter Row cytometry. the light scattering properties of cysts acid trophozoites were studied. The cytoplasmic granularity, reflected by the side scatter of light, was proportional to DNA content of trophozoites, whereas cysts with similar DNA contents showed heterogeneity in their cytoplasmic granularity. Dynamic changes in the intracellular calcium pools were observed during differentiation of trophozoites to cysts. Comparison of E. invadens and Entamoeba histolytica cell cycles suggest that both organisms may have similar regulatory processes during cell division and differentiation. Since E. histolytica cannot be induced to encyst in axenic culture, analysis of the E. invadens cell cycle during encystation may be useful for identifying homologous processes in E.histolytica. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：277 / 285

页数：9

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