Inhibition of protein crystallization by evolutionary negative design

被引：69

作者：

Doye, JPK ^{[1
]}

Louis, AA ^{[1
]}

Vendruscolo, M ^{[1
]}

机构：

[1] Univ Cambridge, Chem Lab, Cambridge CB2 1EW, England

来源：

PHYSICAL BIOLOGY | 2004年 / 1卷 / 1-2期

关键词：

D O I：

10.1088/1478-3967/1/1/P02

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Why are proteins so hard to crystallize? We propose an 'evolutionary negative design' principle to explain this difficulty. Proteins have evolved to avoid crystallization because crystallization compromises the viability of the cell. Evolutionary negative design is supported by much evidence in the literature, including the effect of mutations on the crystallizability of a protein, the correlations found in the properties of crystal contacts in bioinformatics databases, and the positive use of protein crystallization by bacteria and viruses.

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页码：P9 / P13

页数：5

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