Aqueous two-phase partitioning sample preparation prior to liquid chromatography of hydrophilic drugs in blood

被引：31

作者：

Agasoster, T ^{[1
]}

机构：

[1] Univ Tromso, Dept Pharm, N-9037 Tromso, Norway

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 716卷 / 1-2期

关键词：

hydrophilic drugs; aqueous two-phase systems; partitioning;

D O I：

10.1016/S0378-4347(98)00293-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The technique of aqueous two-phase partitioning is investigated as a sample preparation procedure prior to LC determination of drugs in blood. In the extraction 500 mu l Of whole blood is added to 2.00 g of PEG 300 and 18.0 g of phosphate buffer. The phases are mixed by stirring and allowed to separate before the clear lower salt phase is drained out using a specially designed glass adapter. After filtration 20 mu l is injected into the LC system. When validated for the X-ray contrast medium iohexol R.S.D. was 2.0% and 1.2% at 10 mu g/ml and 100 mu g/ml of iohexol in blood, respectively. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：293 / 298

页数：6

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