Diverse environmental cues transiently regulate OsOPR1 of the "octadecanoid pathway" revealing its importance in rice defense/stress and development

被引:74
作者
Agrawal, GK
Jwa, NS
Shibato, J
Han, O
Iwahashi, H
Rakwal, R
机构
[1] Bio Resource Res & Dev Co Pvt Ltd, Kathmandu, Nepal
[2] Sejong Univ, Coll Nat Sci, Dept Mol Biol, Seoul 143747, South Korea
[3] AIST, Tsukuba, Ibaraki 3058566, Japan
[4] Chonnam Natl Univ, Dept Genet Engn, Biotechnol Res Inst, Kwangju 500757, South Korea
关键词
defense/stress response; elicitors; growth; Oryza sativa L. cv. Nipponbare; oxo-phytodienoic acid reductase 1;
D O I
10.1016/j.bbrc.2003.09.123
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previously, ve reported rice (Oryza sativa L. japonica type cv. Nipponbare) allene oxide synthase (OsAOS) and cyclase (OsAOC) genes of the octadecanoid pathway. Here we have isolated a 12-oxo-phytodienoic acid reductase gene, called OsOPR1, encoding the last committed enzymatic step on the octadecanoid pathway leading to jasmonic acid (JA) biosynthesis. OsOPR1 encodes a 380 amino acid long polypeptide with a predicted molecular mass of 42465.02 and pI of 5.79, and belongs to a gene family in the rice genome. Transcriptional profiling using our established two-week-old rice seedling model system against a variety of environmental factors such as wounding, global signals (including JA), osmotic stresses, heavy metals, UV-C irradiation, fungal elicitor, protein phosphatase inhibitors, and gaseous pollutants indicated that OsOPR1 transcript was rapidly, transiently and differentially up-regulated within 30 min in leaves. Surprisingly, co-application of signaling molecules JA, salicylate and ethylene, resulted in a massive accumulation of the OsOPR1 transcript at 30min and remained elevated with time, a new observation. Furthermore, transient expression of OsOPR1, most likely regulated by a de novo synthesized negative trans-acting factor(s), was evidenced by the use of cycloheximide. Finally, the endogenous OsOPR1 expression varied with the stage of plant development. These results strongly suggest a regulatory role for OsOPR1 in rice plant defense/stress response pathway(s) and reproduction. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:1073 / 1082
页数:10
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