Entamoeba histolytica:: Alterations in EhRabB protein in a phagocytosis deficient mutant correlate with the Entamoeba dispar RabB sequences

被引：11

作者：

Guzmán-Medrano, R ^{[1
]}

Castillo-Juárez, BA ^{[1
]}

García-Pérez, RM ^{[1
]}

Salas-Casas, A ^{[1
]}

Orozco, E ^{[1
]}

Rodríguez, MA ^{[1
]}

机构：

[1] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Patol Expt, Mexico City 07000, DF, Mexico

来源：

EXPERIMENTAL PARASITOLOGY | 2005年 / 110卷 / 03期

关键词：

descriptors and abbreviations; Entamoeba histolytica; Entamoeba dispar; EhRabB; Rab GTPase; pliagocytosis-deficient mutant;

D O I：

10.1016/j.exppara.2005.03.002

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

We analyzed the expression and location of EhRabB in clone L-6, a phagocytosis-deficient mutant of Entamoeba histolytica, in comparison with the wild-type clone A. Intriguingly, trophozoites of clone L-6 express more EhRabB than those of clone A. However, the majority of EhRabB-containing vesicles remained in the cytoplasm of clone L-6 during phagocytosis. To investigate molecular alterations in EhRabB of clone L-6 we compared the EhrabB gene sequences from clones L-6 and A. We also isolated, sequenced and compared the RabB protein of Entamoeba dispar. Results showed that EhrabB gene of clone L-6 is 98.2 and 94.1% identical to rabB genes of E. dispar and clone A, respectively. The rabB genes from clone A and E dispar have 92.2% identity. Four out of five amino acids changes in RabB proteins of clone L-6 and E dispar are shared. These changes may alter the binding of effector proteins and the specific subcellular location of EhRabB. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：259 / 264

页数：6

共 23 条

[11] Ras/Rap effector specificity determined by charge reversal [J].