Peroxidase activity and isoenzymes in the culture medium of NaCl adapted tomato suspension cells

被引:90
作者
Sancho, MA
deForchetti, SM
Pliego, F
Valpuesta, V
Quesada, MA
机构
[1] UNIV MALAGA,DEPT BIOL VEGETAL,E-29071 MALAGA,SPAIN
[2] UNIV MALAGA,DEPT BIOQUIM & BIOL MOLEC,E-29071 MALAGA,SPAIN
关键词
lignin; Lycopersicon esculentum; salt-stress; peroxidase isozymes;
D O I
10.1007/BF00048195
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The medium of tomato (Lycopersicon esculentum) cells adapted to grow in the presence of 15 g 1(-1) NaCl had a higher peroxidase activity than the medium of an unadapted tomato cell line. When the adapted cells were cultured in a medium without NaCl, the value found for peroxidase activity was intermediate. The increase in peroxidase activity was parallel to an increase of lignin-like compounds in the cell walls, as well as to an increased content or appearance of neutral and basic peroxidase isoenzymes. Apparently, the high values of peroxidase activity in the medium of the salt-adapted cells reflect the changed mechanical properties of the cell wall which, in turn, could be related to the salt adaptation process.
引用
收藏
页码:161 / 167
页数:7
相关论文
共 35 条
[31]   CELL-WALL STRUCTURE IN CELLS ADAPTED TO GROWTH ON THE CELLULOSE-SYNTHESIS INHIBITOR 2,6-DICHLOROBENZONITRILE - A COMPARISON BETWEEN 2 DICOTYLEDONOUS PLANTS AND A GRAMINEOUS MONOCOT [J].
SHEDLETZKY, E ;
SHMUEL, M ;
TRAININ, T ;
KALMAN, S ;
DELMER, D .
PLANT PHYSIOLOGY, 1992, 100 (01) :120-130
[32]  
STERJIADES R, 1993, PLANTA, V190, P75, DOI 10.1007/BF00195678
[33]   AN IMMUNOLOGICAL STUDY OF PEROXIDASE RELEASE BY CULTURED PEANUT CELLS [J].
VANHUYSTEE, RB ;
LOBARZEWSKI, J .
PLANT SCIENCE LETTERS, 1982, 27 (01) :59-67
[34]  
WINICOV I, 1991, PLANTA, V183, P478, DOI 10.1007/BF00194267
[35]   OXIDATION OF TYROSINE BY PEROXIDASE ISOZYMES DERIVED FROM PEANUT SUSPENSION-CULTURE MEDIUM AND BY ISOLATED CELL-WALLS [J].
ZHENG, XH ;
VANHUYSTEE, RB .
PLANT CELL TISSUE AND ORGAN CULTURE, 1991, 25 (01) :35-43