Isolation, nucleotide sequence and expression of a cDNA encoding feline granulocyte colony-stimulating factor

被引：4

作者：

Dunham, SP ^{[1
]}

Onions, DE ^{[1
]}

机构：

[1] Univ Glasgow, Dept Vet Pathol, Retrovirus Res Lab, Glasgow G61 1QH, Lanark, Scotland

来源：

CYTOKINE | 2001年 / 14卷 / 06期

基金：

英国惠康基金;

关键词：

feline G-CSF; polymerase chain reaction; nucleotide sequence; cytokine; bacterial expression;

D O I：

10.1006/cyto.2001.0910

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding feline granulocyte colony stimulating factor (fG-CSF) was cloned from alveolar macrophages using the reverse transcriptase-polymerase chain reaction. The cDNA is 949 bp in length and encodes a predicted mature protein of 174 amino acids. Recombinant fG-CSF was expressed as a glutathione S-transferase fusion and purified by affinity chromatography. Biological activity of the recombinant protein was demonstrated using the murine myeloblastic cell line GNFS-60, which showed an ED50 for fG-CSF of approximately 2 ng/ml. (C) 2001 Academic Press.

引用

页码：347 / 351

页数：5

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