CLONING AND SEQUENCING OF AN OVINE GRANULOCYTE-COLONY-STIMULATING FACTOR CDNA

被引：5

作者：

OBRIEN, PM ^{[1
]}

SEOW, HF ^{[1
]}

ROTHEL, JS ^{[1
]}

WOOD, PR ^{[1
]}

机构：

[1] CSIRO,DIV ANIM HLTH,ANIM HLTH RES LAB,PARKVILLE,VIC 3052,AUSTRALIA

来源：

DNA SEQUENCE | 1994年 / 4卷 / 05期

关键词：

NEUTROPHIL; NUCLEOTIDE SEQUENCE; OVINE G-CSF; PCR;

D O I：

10.3109/10425179409020862

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA encoding an ovine (ov) granulocyte colony-stimulating factor (C-CSF) was cloned by polymerase chain reaction, using primers based on the 5' and 3' nucleotide (nt) sequence of the bovine (bov) G-CSF cDNA. RNA was isolated from IFN-gamma and LPS-stimulated alveolar macrophages. The isolated oVG-CSF cDNA is 522 bp in length and does not include coding sequence for a secretory signal peptide. The nt sequence is 95% identical to the bovG-CSF nt sequence, and includes conservation of 5 cysteine residues. The deduced amino acid (aa) sequence of the mature ovG-CSF protein shares 96%, 82% and 68% homology with the bov, human and murine mature C-CSF proteins, respectively. The estimated molecular weight of the 174 aa C-CSF protein is 18.8 kilodaltons.

引用

页码：339 / 342

页数：4

共 26 条

[1]

BOONE TC, 1989, Patent No. 10932

[2] PRODUCTION OF COLONY-STIMULATING FACTORS (CSFS) DURING INFECTION - SEPARATE DETERMINATIONS OF MACROPHAGE-CSF, GRANULOCYTE-CSF, GRANULOCYTE-MACROPHAGE-CSF, AND MULTI-CSF [J].

CHEERS, C ;

HAIGH, AM ;

KELSO, A ;

METCALF, D ;

STANLEY, ER ;

YOUNG, AM .

INFECTION AND IMMUNITY, 1988, 56 (01) :247-251

[3] INVIVO STIMULATION OF GRANULOPOIESIS BY RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR [J].

COHEN, AM ;

ZSEBO, KM ;

INOUE, H ;

HINES, D ;

BOONE, TC ;

CHAZIN, VR ;

TSAI, L ;

RITCH, T ;

SOUZA, LM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (08) :2484-2488

[4] HEMATOLOGIC AND BONE-MARROW CHANGES AFTER SHORT-TERM AND LONG-TERM ADMINISTRATION OF 2 RECOMBINANT BOVINE GRANULOCYTE COLONY-STIMULATING FACTORS [J].

CULLOR, JS ;

SMITH, W ;

ZINKL, JG ;

DELLINGER, JD ;

BOONE, T .

VETERINARY PATHOLOGY, 1992, 29 (06) :521-527

[5] RAPID CLONING OF HLA-A,B CDNA BY USING THE POLYMERASE CHAIN-REACTION - FREQUENCY AND NATURE OF ERRORS PRODUCED IN AMPLIFICATION [J].

ENNIS, PD ;

ZEMMOUR, J ;

SALTER, RD ;

PARHAM, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2833-2837

[6]

Haig D. M., 1990, Parasites: immunity and pathology. The consequences of parasitic infection in mammals., P42

[7] COLONY STIMULATING FACTORS, CYTOKINES AND MONOCYTE-MACROPHAGES - SOME CONTROVERSIES [J].

HAMILTON, JA .

IMMUNOLOGY TODAY, 1993, 14 (01) :18-24

[8] THE STRUCTURE OF GRANULOCYTE-COLONY-STIMULATING FACTOR AND ITS RELATIONSHIP TO OTHER GROWTH-FACTORS [J].

HILL, CP ;

OSSLUND, TD ;

EISENBERG, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (11) :5167-5171

[9] RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR ENHANCES SUPEROXIDE RELEASE IN HUMAN-GRANULOCYTES STIMULATED BY THE CHEMOTACTIC PEPTIDE [J].

KITAGAWA, S ;

YUO, A ;

SOUZA, LM ;

SAITO, M ;

MIURA, Y ;

TAKAKU, F .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 144 (03) :1143-1146

[10]

LOPEZ AF, 1983, J IMMUNOL, V131, P2983

← 1 2 3 →