In mouse tissues two variants of the transient receptor potential (canonical) (TRPC) 4 protein are expressed: the "full-length" TRPC4 protein and a slightly smaller variant, called TRPC4 Delta(761-864), which lacks 84 amino acid residues. Although the presence of mRNA encoding the TRPC4 protein in mammalian cells and the detection of the heterologously expressed TRPC4 protein by Western blot analysis have been reported, the unequivocal detection of endogenous TRPC4 proteins has proven difficult. In the present study we compared polyclonal antibodies for the detection of TRPC4 proteins in mouse tissues and monitored their specificity and reliability by analysing corresponding tissues from TRPC4-deficient mice. In addition we introduced a procedure that allows us to estimate the amount of TRPC4 protein expressed in a single cell. Using this technique it appears that the amount of TRPC4 protein expressed stably in HEK 293 cells is at least fourfold higher than the amount of TRPC4 protein expressed endogenously in the bovine adrenocortical cell line SBAC.
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Corey D.P.
;
Garcia-Añoveros J.
论文数: 0引用数: 0
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机构:
Department of Anesthesiology, NW Univ. Institute for Neurosciences, ChicagoDepartment of Neurobiology, Harvard Medical School, Boston
Garcia-Añoveros J.
;
Holt J.R.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neuroscience, Univ. of Virginia School of Medicine, CharlottesvilleDepartment of Neurobiology, Harvard Medical School, Boston
Holt J.R.
;
Kwan K.Y.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Kwan K.Y.
;
Lin S.-Y.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Department of Biology, Massachusetts Inst. of Technology, CambridgeDepartment of Neurobiology, Harvard Medical School, Boston
Lin S.-Y.
;
Vollrath M.A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Vollrath M.A.
;
Amalfitano A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Pediatrics, Division of Medical Genetics, Duke University Medical Center, DurhamDepartment of Neurobiology, Harvard Medical School, Boston
Amalfitano A.
;
Cheung E.L.-M.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Cheung E.L.-M.
;
Derfler B.H.
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h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Derfler B.H.
;
Duggan A.
论文数: 0引用数: 0
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机构:
Department of Anesthesiology, NW Univ. Institute for Neurosciences, ChicagoDepartment of Neurobiology, Harvard Medical School, Boston
Duggan A.
;
Géléoc G.S.G.
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机构:
Department of Neuroscience, Univ. of Virginia School of Medicine, CharlottesvilleDepartment of Neurobiology, Harvard Medical School, Boston
Géléoc G.S.G.
;
Gray P.A.
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h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Dana-Farber Cancer Institute, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Gray P.A.
;
Hoffman M.P.
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机构:
Matrix and Morphogenesis Unit, NIDCR, NIH, BethesdaDepartment of Neurobiology, Harvard Medical School, Boston
Hoffman M.P.
;
Rehm H.L.
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机构:
Laboratory for Molecular Medicine, Harvard-Partners Genome Center, CambridgeDepartment of Neurobiology, Harvard Medical School, Boston
Rehm H.L.
;
Tamasauskas D.
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h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Tamasauskas D.
;
Zhang D.-S.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Corey D.P.
;
Garcia-Añoveros J.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Anesthesiology, NW Univ. Institute for Neurosciences, ChicagoDepartment of Neurobiology, Harvard Medical School, Boston
Garcia-Añoveros J.
;
Holt J.R.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neuroscience, Univ. of Virginia School of Medicine, CharlottesvilleDepartment of Neurobiology, Harvard Medical School, Boston
Holt J.R.
;
Kwan K.Y.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Kwan K.Y.
;
Lin S.-Y.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Department of Biology, Massachusetts Inst. of Technology, CambridgeDepartment of Neurobiology, Harvard Medical School, Boston
Lin S.-Y.
;
Vollrath M.A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Vollrath M.A.
;
Amalfitano A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Pediatrics, Division of Medical Genetics, Duke University Medical Center, DurhamDepartment of Neurobiology, Harvard Medical School, Boston
Amalfitano A.
;
Cheung E.L.-M.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Cheung E.L.-M.
;
Derfler B.H.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Derfler B.H.
;
Duggan A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Anesthesiology, NW Univ. Institute for Neurosciences, ChicagoDepartment of Neurobiology, Harvard Medical School, Boston
Duggan A.
;
Géléoc G.S.G.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neuroscience, Univ. of Virginia School of Medicine, CharlottesvilleDepartment of Neurobiology, Harvard Medical School, Boston
Géléoc G.S.G.
;
Gray P.A.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Dana-Farber Cancer Institute, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Gray P.A.
;
Hoffman M.P.
论文数: 0引用数: 0
h-index: 0
机构:
Matrix and Morphogenesis Unit, NIDCR, NIH, BethesdaDepartment of Neurobiology, Harvard Medical School, Boston
Hoffman M.P.
;
Rehm H.L.
论文数: 0引用数: 0
h-index: 0
机构:
Laboratory for Molecular Medicine, Harvard-Partners Genome Center, CambridgeDepartment of Neurobiology, Harvard Medical School, Boston
Rehm H.L.
;
Tamasauskas D.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston
Tamasauskas D.
;
Zhang D.-S.
论文数: 0引用数: 0
h-index: 0
机构:
Department of Neurobiology, Harvard Medical School, Boston
Howard Hughes Medical Institute, Harvard Medical School, BostonDepartment of Neurobiology, Harvard Medical School, Boston