A new method for the determination of selected PAHs in coffee brew samples by HPLC with fluorimetric detection and solid-phase extraction

被引：32

作者：

Kayali-Sayadi, MN ^{[1
]}

Rubio-Barroso, S ^{[1
]}

Cuesta-Jimenez, MP ^{[1
]}

Polo-Díez, LM ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, E-28040 Madrid, Spain

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1999年 / 22卷 / 04期

关键词：

D O I：

10.1081/JLC-100101686

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid analytical method is proposed for the determination of PAHs (fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[e]pyrene, benzo[a]pyrene, dibenzo[a]anthracene and benzo[g,h,i]perylene) in coffee brew samples. The method is based on solid phase extraction with Sep-Pak Vac tC-18 cartridges and elution with ethyl ether. The extracts were analyzed by RP-HPLC with a Green PAH column and fluorescence detection under an acetonitrile-water mobile phase gradient. Some PAHs were detected in the samples studied and their mean contents were 1.65-2.87 ng/L coffee brew. The relative standard deviations were in the range 2-13% for four replicates.

引用

页码：615 / 627

页数：13

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