Placental protein 13 (PP13/galectin-13) undergoes lipid raft-associated subcellular redistribution in the syncytiotrophoblast in preterm preeclampsia and HELLP syndrome

被引：52

作者：

Balogh, Andrea ^{[2
]}

Pozsgay, Judit ^{[3
]}

Matko, Janos ^{[2
,3
]}

Dong, Zhong

Kim, Chong Jai

Varkonyi, Tibor ^{[4
]}

Sammar, Marei ^{[5
]}

Rigo, Janos, Jr. ^{[4
]}

Meiri, Hamutal ^{[6
]}

Romero, Roberto ^{[1
]}

Papp, Zoltan ^{[4
]}

Than, Nandor Gabor ^{[1
,4
]}

机构：

[1] Wayne State Univ, Ctr Mol Med & Genet, Detroit, MI 48201 USA

[2] Hungarian Acad Sci, Immunol Res Grp, Budapest, Hungary

[3] Eotvos Lorand Univ, Dept Immunol, H-1117 Budapest, Hungary

[4] Semmelweis Univ, Dept Obstet & Gynecol 1, Budapest, Hungary

[5] ORT Braude Coll, Dept Biotechnol Engn, Karmiel, Israel

[6] TeleMarpeh Ltd, Tel Aviv, Israel

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2011年 / 205卷 / 02期

关键词：

alarmin; brush border membrane; damage-associated molecular pattern (DAMP); danger signal; galectin; nonclassical" secretory pathway; ELEVATED LIVER-ENZYMES; NORMAL-PREGNANCY; MEMBRANE MICRODOMAINS; CLINICAL-SIGNIFICANCE; SOLUBLE ENDOGLIN; BRUSH-BORDERS; IMMUNE-SYSTEM; ANNEXIN II; IN-VITRO; GALECTINS;

D O I：

10.1016/j.ajog.2011.03.023

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: To investigate placental protein 13 (PP13) localization in relation to cytoskeleton and lipid rafts in preeclampsia and HELLP syndrome. STUDY DESIGN: Placental cryosections from patients with preeclampsia and HELLP, and controls were stained for PP13, actin, PLAP (lipid raft marker), and CD71 (nonraft marker). BeWo cells exposed to stress conditions were stained for PP13 and actin. Protein localizations were investigated by confocal microscopy, PP13 concentrations by ELISA. RESULTS: PP13-actin colocalization was increased in syncytiotrophoblast juxtamembrane regions in term/preterm preeclampsia and HELLP. PP13-CD71 colocalization was decreased and PP13-PLAP proximity was increased in preterm but not term preeclampsia and HELLP. PP13-release from BeWo cells was inhibited by cytoskeleton disruption, and augmented by Ca2+-influx and ischemic stress. CONCLUSION: The actin cytoskeleton, probably in connection with lipid rafts, controls trophoblastic "nonclassical" PP13 export. PP13 is released from the syncytiotrophoblast in preterm preeclampsia and HELLP, mimicked in BeWo cells by ischemic stress, suggesting PP13 is a placental alarmin.

引用

页码：156.e1 / 156.e14

页数：14

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