Under flow impedimetric measurements using magnetic particles for label-free detection affinity target

被引:8
作者
Hassen, Walid Mohamed [1 ]
Angnes, Lucio [3 ]
Abdelghani, Adnane [2 ]
Bessueille, Francois [1 ]
Leonard, Didier [1 ]
Jaffrezic-Renault, Nicole [1 ]
机构
[1] Univ Lyon 1, CNRS, Analyt Sci Lab, UMR 5180, F-69622 Villeurbanne, France
[2] IPEST, Unite Rech Phys Semicond & Capteurs, Tunis 2070, Tunisia
[3] Univ Sao Paulo, Inst Quim, BR-05508900 Sao Paulo, Brazil
来源
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 5-6期
关键词
under flow impedance spectroscopy; magnetic particles; streptavidin; biotin; FTIR; AFM;
D O I
10.1016/j.msec.2007.10.041
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The necessity to adapt sensors based on electrochemical techniques for high throughput analysis control increases the interest to develop new analytical systems able to perform measurements under buffer now. In this report we explored the possibility of employing a new system to make impedimetric measurements to detect the interaction between proteins and small molecules. The well-known biotin-streptavidin interaction was adopted to evaluate the proposed assembly. This system allows us to perform experiments under flow. Magnetic beads functionalized with streptavidin were used and first characterized using AFM and FTIR. Non-faradic impedance spectroscopy allowed the detection of the biotin-streptavidin interaction. Using our new system and under a flow of PBS buffer, 5 10-5 M of biotin was detected with a stable signal. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:820 / 825
页数:6
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