In vivo detection of myelin proteins in cervical lymph nodes of MS patients using ultrasound-guided fine-needle aspiration cytology

被引：77

作者：

Fabriek, BO

Zwernmer, JNP

Teunissen, CE

Dijkstra, CD

Polman, CH

Laman, JD

Castelijns, JA

机构：

[1] VU Med Ctr, Dept Mol Cell Biol & Immunol, NL-1007 MB Amsterdam, Netherlands

[2] VU Med Ctr, Dept Neurol, Amsterdam, Netherlands

[3] Erasmus Univ, Med Ctr, Dept Immunol, NL-3000 DR Rotterdam, Netherlands

[4] Erasmus Univ, Med Ctr, MS Ctr ErasMS, NL-3000 DR Rotterdam, Netherlands

[5] VU Med Ctr, Dept Radiol, Rotterdam, Netherlands

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2005年 / 161卷 / 1-2期

关键词：

cervical lymph node; multiple sclerosis; brain; in vivo; myelin proteins;

D O I：

10.1016/j.jneuroim.2004.12.018

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cervical lymph nodes (CLN) have been described to be the first lymphoid draining site of the brain. In this study we used ultrasound guided fine needle aspiration cytology (USgFNAC) to obtain cells, in vivo, from non-enlarged CLN of multiple sclerosis (MS) patients and HCs (HC), and investigated whether myelin proteins could be detected. Macrophages containing myelin basic protein (MBP) and proteolipid protein (PLP) were found in CLN of all MS patients, whereas these could only be detected in a minority of HC. This novel approach allows investigation of the first draining site of the brain for in vivo analysis of the immune regulation of MS. (C) 2005 Elsevier B.V. All rights reserved.

引用

页码：190 / 194

页数：5

共 16 条

[11]

Phillips MJ, 1997, J PATHOL, V182, P457

[12] A putative mechanism of demyelination in multiple sclerosis by a proteolytic enzyme, calpain [J].