Inhibition of telomerase activity of melanoma cells in vitro by antisense oligonucleotides

被引：75

作者：

Glukhov, AI

Zimnik, OV

Gordeev, SA

Severin, SE ^{[1
]}

机构：

[1] Moscow Res Inst Med Ecol, Moscow 113149, Russia

[2] IM Sechenov Med Acad, Moscow 113149, Russia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.8801

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of antisense oligonucleotides complementary to the RNA component of human telomerase on telomerase activity in cell extracts of the melanoma cell line SK-Mel-28 has been studied. It has been shown that the antisense oligonucleotide complementary to the hTR. component in the region of the template synthesis of telomeric repeats is the most efficient inhibitor of telomerase activity in comparison with other antisense oligonucleotides. Pronounced inhibition of telomerase activity was observed at the oligonucleotide (Tel P5) concentration in the reaction mixture of about 5 nM, Complete inhibition of the enzyme occurs at the oligonucleotide concentration in the sample of about 20 nM. (C) 1998 Academic Press.

引用

页码：368 / 371

页数：4

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[21] A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES [J].