Effect of pyrene on denitrification activity and abundance and composition of denitrifying community in an agricultural soil

被引：87

作者：

Guo, Guang-Xia ^{[1
]}

Deng, Huan ^{[1
]}

Qiao, Min ^{[1
]}

Mu, Yu-Jing ^{[1
]}

Zhu, Yong-Guan ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, State Key Lab Reg & Urban Ecol, Res Ctr Ecoenvironm Sci, Beijing 100085, Peoples R China

[2] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2011年 / 159卷 / 07期

关键词：

Denitrifiers; EC50; Phylogenetic analysis; Temporal shifts; Diversity; POLYCYCLIC AROMATIC-HYDROCARBONS; REDUCTASE GENES NIRK; RICE PADDY SOIL; NITROUS-OXIDE; MICROBIAL COMMUNITIES; HEAVY-METALS; NITRIC-OXIDE; DIVERSITY; BACTERIAL; DEGRADATION;

D O I：

10.1016/j.envpol.2011.03.035

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Toxicity of pyrene on the denitrifiers was studied by spiking an agricultural soil with pyrene to a series of concentrations (0-500 mg kg(-1)) followed by dose response and dynamic incubation experiments. Results showed a positive correlation between potential denitrification activity and copy numbers of denitrifying functional genes (nirK, nirS and nosZ), and were both negatively correlated with pyrene concentrations. Based on the comparison of EC50 values, denitrifiers harboring nirK, nirS or nosZ gene were more sensitive than denitrification activity, and denitrifiers harboring nirS gene were more sensitive than that harboring nirK or nosZ genes. Seven days after spiking with EC50 concentration of pyrene, denitrifiers diversity decreased and community composition changed in comparison with the control. Phylogenetic analyses of three genes showed that the addition of pyrene increased the proportion of Bradyrhizobiaceae, Rhodospirillales, Burkholderiales and Pseudomonadales. Some species belonging to these groups were reported to be able to degrade PAHs. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1886 / 1895

页数：10

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