Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor

被引：667

作者：

Xiao, Y

Lubin, AA

Heeger, AJ

Plaxco, KW ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA

[5] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Santa Barbara, CA 93106 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 34期

关键词：

aptamers; biosensors; DNA structures; electron transfer; thrombin;

D O I：

10.1002/anie.200500989

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin-binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5456 / 5459

页数：4

共 48 条

[21] A review of molecular recognition technologies for detection of biological threat agents
Iqbal, SS
Mayo, MW
Bruno, JG
Bronk, BV
Batt, CA
Chambers, JP
[J]. BIOSENSORS & BIOELECTRONICS, 2000, 15 (11-12) : 549 - 578
[22] Folding of the thrombin aptamer into a G-quadruplex with Sr2+:: Stability, heat, and hydration
Kankia, BI
Marky, LA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (44) : 10799 - 10804
[23] GENERAL EXPRESSION OF THE LINEAR POTENTIAL SWEEP VOLTAMMOGRAM IN THE CASE OF DIFFUSIONLESS ELECTROCHEMICAL SYSTEMS
LAVIRON, E
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1979, 101 (01) : 19 - 28
[24] A fiber-optic microarray biosensor using aptamers as receptors
Lee, M
Walt, DR
[J]. ANALYTICAL BIOCHEMISTRY, 2000, 282 (01) : 142 - 146
[25] Li Jun, 2004, Current Proteomics, V1, P315
[26] Molecular aptamer beacons for real-time protein recognition
Li, JWJ
Fang, XH
Tan, WH
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 292 (01) : 31 - 40
[27] An aptamer-based quartz crystal protein biosensor
Liss, M
Petersen, B
Wolf, H
Prohaska, E
[J]. ANALYTICAL CHEMISTRY, 2002, 74 (17) : 4488 - 4495
[28] Louis B., 1992, NATURE, V355, P564
[29] THROMBIN-BINDING DNA APTAMER FORMS A UNIMOLECULAR QUADRUPLEX STRUCTURE IN SOLUTION
MACAYA, RF
SCHULTZE, P
SMITH, FW
ROE, JA
FEIGON, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) : 3745 - 3749
[30] Development of biosensors with aptamers as bio-recognition element: the case of HIV-1 Tat protein
Minunni, M
Tombelli, S
Gullotto, A
Luzi, E
Mascini, M
[J]. BIOSENSORS & BIOELECTRONICS, 2004, 20 (06) : 1149 - 1156

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