A DNA polymerase stop assay for G-quadruplex-interactive compounds

被引:220
作者
Han, HY
Hurley, LH
Salazar, M [1 ]
机构
[1] Univ Texas, Coll Pharm, Div Med Chem, Austin, TX 78712 USA
[2] Univ Texas, Coll Pharm, Program Mol Biol, Austin, TX 78712 USA
[3] Univ Texas, Coll Pharm, Drug Dynam Inst, Austin, TX 78712 USA
关键词
D O I
10.1093/nar/27.2.537
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have developed and characterized an assay for G-quadruplex-interactive compounds that makes use of the fact that G-rich DNA templates present obstacles to DNA synthesis by DNA polymerases. Using Tao DNA polymerase and the G-quadruplex binding 2,6-diamidoanthraquinone BSU-1051, we find that BSU-1051 leads to enhanced arrest of DNA synthesis in the presence of K+ by stabilizing an intramolecular G-quadruplex structure formed by four repeats of either TTGGGG or TTAGGG in the template strand. The data provide additional evidence that BSU-1051 modulates telomerase activity by stabilization of telomeric G-quadruplex DNA and point to a polymerase arrest assay as a sensitive method for screening for G-quadruplex-interactive agents with potential clinical utility.
引用
收藏
页码:537 / 542
页数:6
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