BAFF/SAFF-R involved in antibodies production of rats with collagen-induced arthritis via PI3K-Akt-mTOR signaling and the regulation of paeoniflorin

被引：61

作者：

Li, Pei-Pei ^{[1
]}

Liu, Dan-Dan ^{[1
]}

Liu, Yun-Jie ^{[1
]}

Song, Shan-Shan ^{[1
]}

Wang, Qing-Tong ^{[1
]}

Chang, Yan ^{[1
]}

Wu, Yu-Jing ^{[1
]}

Chen, Jing-Yu ^{[1
]}

Zhao, Wen-Di ^{[1
]}

Zhang, Ling-Ling ^{[1
]}

Wei, Wei ^{[1
]}

机构：

[1] Anhui Med Univ, Inst Clin Pharmacol, Key Lab Antiinflammatory & Immunopharmacol, Educ Minist China, Hefei 230032, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2012年 / 141卷 / 01期

基金：

中国国家自然科学基金;

关键词：

PI3K/Akt/mTOR; Signaling; BAFF/BAFF-R; Collagen induced arthritis; Paeoniflorin; INDUCED IMMUNE TOLERANCE; LYMPH-NODE LYMPHOCYTES; CELL ACTIVATION FACTOR; B-CELL; RHEUMATOID-ARTHRITIS; RECEPTOR DESENSITIZATION; ADJUVANT ARTHRITIS; TACI-IG; BAFF-R; MICE;

D O I：

10.1016/j.jep.2012.02.034

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ethnopharmacological relevance: Paeoniflorin (Pae) is extracted from the root of paeonia lactiflora which have attracted attention for anti-rheumatic and immune modulating properties. Aim of the study: To investigate the role of PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R in antibodies production and the regulation of Pae on the signaling pathway in rats with collagen-induced arthritis (CIA). Materials and methods: CIA rats were randomly separated into different groups and treated with Pae (25, 100 mg/kg) from day 18 to day 38 after immunization. The effects of Pae on B lymphocytes of CIA rats were evaluated by the levels of BAFF, anti-CII antibody, IgA, IgG and IgM, and the expressions of BAFF-R, P13K, p-Akt and mTOR. Results: In CIA rats, the levels of anti-CII antibody, IgA, IgG and IgM in serum enhanced, BAFF, BAFF-R, PI3K, p-Akt and mTOR were highly expressed. Pae (100 mg/kg) obviously decreased arthritis score, relieved ankle and paw swelling, improved spleen histopathology in CIA rats, decreased the levels of IgA, IgM, IgG and anti-CII antibody, and significantly decreased the expressions of BAFF, BAFF-R, PI3K, p-Akt and mTOR. Conclusion: PI3K/Akt/mTOR signaling mediated by BAFF/BAFF-R participates in antibodies production by B lymphocytes of CIA rats. Pae had therapeutic effects on rats with CIA. These effects might be relative to regulating PI3K/Akt/mTOR signal mediated by BAFF/BAFF-R, and down regulate the antibodies production further. (c) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：290 / 300

页数：11

共 30 条

[11] Therapeutic effects of TACI-Ig on collagen-induced arthritis by regulating T and B lymphocytes function in DBA/1 mice [J].

Liu, Yunjie ;

Zhang, Ling Ling ;

Wu, Yingqi ;

Tong, Tong ;

Zhao, Wendi ;

Li, Peipei ;

Huang, Min ;

Wang, Wenxiang ;

Fang, Jianmin ;

Wei, Wei .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2011, 654 (03) :304-314

[12] Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control [J].

Loewith, R ;

Jacinto, E ;

Wullschleger, S ;

Lorberg, A ;

Crespo, JL ;

Bonenfant, D ;

Oppliger, W ;

Jenoe, P ;

Hall, MN .

MOLECULAR CELL, 2002, 10 (03) :457-468

[13]

Mackay Fabienne, 2005, V8, P243

[14] Systemic autoimmunity in BAFF-R-mutant A/WySnJ strain mice [J].

Mayne, Christopher G. ;

Arnanna, Ian J. ;

Nashold, Faye E. ;

Hayes, Colleen E. .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2008, 38 (02) :587-598

[15]

Nq L.G., 2004, J IMMUNOL, V173, P807

[16] BAFF activates Akt and Erk through BAFF-R in an IKK1-dependent manner in primary mouse B cells [J].

Otipoby, Kevin L. ;

Sasaki, Yoshiteru ;

Schmidt-Supprian, Marc ;

Patke, Alina ;

Gareus, Ralph ;

Pasparakis, Manolis ;

Tarakhovsky, Alexander ;

Rajewsky, Klaus .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (34) :12435-12438

[17] BAFF controls B cell metabolic fitness through a PKCβ- and Akt-dependent mechanism [J].

Patke, Alina ;

Mecklenbrauker, Ingrid ;

Erdjument-Bromage, Hediye ;

Tempst, Paul ;

Tarakhovsky, Alexander .

JOURNAL OF EXPERIMENTAL MEDICINE, 2006, 203 (11) :2551-2562

[18] The role of collagen antibodies in mediating arthritis [J].

Rowley, Merrill J. ;

Nandakumar, Kutty Selva ;

Holmdahl, Rikard .

MODERN RHEUMATOLOGY, 2008, 18 (05) :429-441

[19] BLyS inhibition eliminates primary B cells but leaves natural and acquired humoral immunity intact [J].

Scholz, Jean L. ;

Crowley, Jenni E. ;

Tomayko, Mary M. ;

Steinel, Natalie ;

O'Neill, Patrick J. ;

Quinn, William J., III ;

Goenka, Radhika ;

Miller, Juli P. ;

Cho, Yun Hee ;

Long, Vatana ;

Ward, Chris ;

Migone, Thi-Sau ;

Shlomchik, Mark J. ;

Cancro, Michael P. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (40) :15517-15522

[20] BLyS and APRIL in rheumatoid arthritis [J].

Seyler, TM ;

Park, YW ;

Takemura, S ;

Bram, RJ ;

Kurtin, PJ ;

Goronzy, JJ ;

Weyand, CM .

JOURNAL OF CLINICAL INVESTIGATION, 2005, 115 (11) :3083-3092

← 1 2 3 →