Separase regulates INCENP-Aurora B anaphase spindle function through Cdc14

被引：213

作者：

Pereira, G

Schiebel, E

机构：

[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England

[2] Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

来源：

SCIENCE | 2003年 / 302卷 / 5653期

关键词：

D O I：

10.1126/science.1091936

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The inner centromere-like protein ( INCENP) forms a complex with the evolutionarily conserved family of Aurora B kinases. The INCENP-Aurora complex helps coordinate chromosome segregation, spindle behavior, and cytokinesis during mitosis. INCENP-Aurora associates with kinetochores in metaphase and with spindle microtubules in anaphase, yet the trigger for this abrupt transfer is unknown. Here we show that the conserved phosphatase Cdc14 regulated the yeast INCENP-Aurora complex, Sli15-Ipl1. Cdc14 dephosphorylated Sli15 and thereby directed the complex to spindles. Activation of Cdc14 by separase was sufficient for Sli15 dephosphorylation and relocalization. Cdc14 not only regulates mitotic exit but also modulates spindle midzone assembly through Sli15-Ipl1.

引用

页码：2120 / 2124

页数：5