Improvement of lactic cell production

被引：18

作者：

Desmons, S ^{[1
]}

Krhouz, H ^{[1
]}

Evrard, P ^{[1
]}

Thonart, P ^{[1
]}

机构：

[1] Fac Univ Sci Agron Gembloux, Ctr Wallon Biol Ind, B-5030 Gembloux, Belgium

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 70-2卷 / 1期

关键词：

L-brevis; freeze-drying; fermentation; accelerated storage test;

D O I：

10.1007/BF02920164

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The production of Lactobacillus brevis has been improved by changing the medium composition and the physiological conditions. The cellular concentration reaches 8.2 x 10(10) cells/mL in 21 h with a fed-batch technique in MRS medium. Freeze-drying has been used as technology for drying the cells and survival rates have been improved with different additives such as glycerol, CaCO3, and skimmed-milk powder up to 70%. A model has been developed to predict the stability of freeze-dried cultures during long-term conservation. This model, based on Arrhenius equation, has been confirmed by experimental data.

引用

页码：513 / 526

页数：14

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