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Localization and characterization of a short isoform of the corticotropin-releasing factor receptor type 2α (CRF2α-tr) in the rat brain

被引：27
作者：
Miyata, I
Shiota, C
Chaki, S
Okuyama, S
Inagami, T
机构：
[1] Vanderbilt Univ, Sch Med, Dept Biochem, Nashville, TN 37232 USA
[2] Taisho Pharmaceut Co Ltd, Med Res Labs, Omiya, Saitama 3308530, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 280卷 / 02期
关键词：
D O I：
10.1006/bbrc.2000.4112
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have recently isolated a cDNA encoding a short isoform of the corticotropin-releasing factor (CRF) receptor subtype, referred to as CRF(2)alpha -tr, from the rat amygdala. The present study determined the localization of the truncated receptor mRNA in the rat brain by in situ hybridization histochemistry. The results showed significant levels of hybridization in the lateral septum, central nucleus of the amygdala, cortico-amygdaloid nucleus, ventrornedial nucleus of the hypothalamus (VMH), and frontal cortex. In the physiological study, antidepressive drugs increased the expression of CRF(2)alpha -tr mRNA and the total binding activity to CRF in the rat amygdala. These findings suggest that CRF(2)alpha -tr may regulate endogeneous CRF release in the amygdala. (C) 2001 Academic Press.
引用
收藏
页码：553 / 557
页数：5
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