MegaPlex PCR: a strategy for multiplex amplification

被引：33

作者：

Meuzelaar, Linda Stroemqvist

Lancaster, Owen

Pasche, J. Paul

Kopal, Guido

Brookes, Anthony J.

机构：

[1] Univ Leicester, Dept Genet, Leicester LE1 7RH, Leics, England

[2] Roche Appl Sci, D-82372 Penzberg, Germany

来源：

NATURE METHODS | 2007年 / 4卷 / 10期

关键词：

D O I：

10.1038/NMETH1091

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair. We demonstrate this method by co-amplifying and genotyping 75 unselected human single-nucleotide polymorphism ( SNP) loci.

引用

页码：835 / 837

页数：3

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