Sensitive and specific method for detecting G protein-coupled receptor mRNAs

被引：14

作者：

Hansen, Arne ^{[1
]}

Chen, Yidong

Inman, Jason M.

Phan, Quang N.

Qi, Zhi-Qing

Xiang, Charlie C.

Palkovits, Miklos

Cherman, Natasha

Kuznetsov, Sergei A.

Robey, Pamela G.

Mezey, Eva

Brownstein, Michael J.

机构：

[1] Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, Bethesda, MD 20892 USA

[2] Natl Inst Neurol Disorder & Stroke, Neurochem Lab, Bethesda, MD 20892 USA

[3] NHGRI, Canc Genet Branch, Bethesda, MD 20892 USA

[4] NIMH, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA

[5] Inst Genom Res, Rockville, MD 20850 USA

[6] Craig Venter Inst, Rockville, MD 20850 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 01期

关键词：

D O I：

10.1038/NMETH977

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

G protein-coupled receptors ( GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mu g of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines. (c) 2007 Nature Publishing Group

引用

页码：35 / 37

页数：3

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