Effect of aggregate size in cell cultures of Saussurea medusa on cell growth and jaceosidin production

被引：28

作者：

Zhao, D ^{[1
]}

Huang, Y ^{[1
]}

Jin, Z ^{[1
]}

Qu, W ^{[1
]}

Lu, D ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China

来源：

PLANT CELL REPORTS | 2003年 / 21卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Saussurea medusa; cell aggregates; jaceosidin production; bioreactor;

D O I：

10.1007/s00299-003-0631-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cell suspension cultures of Saussurea medusa were grown in shake flasks and a 5-l stirred tank bioreactor. Biomass and jaceosidin distribution in cell aggregates of different sizes were investigated during the cultivation period. The results showed that on day 10, jaceosidin accumulation showed an increase with increasing size of the cell aggregate to 4 mm in diameter, with the highest jaceosidin accumulation being 12.2 mg/g. An inverse tendency was observed with cell aggregates larger than 4 mm in diameter, with the lowest accumulation being 3.1 mg/g. However, all of the cell aggregates, despite their size, synthesized almost the same amount of jaceosidin at day 12. Oxygen diffusion limitation and cell-cell contact may explain this behavior. In comparison with cells cultivated in shake flasks, decreased biomass and decreased jaceosidin concentration were observed when the cells were cultivated in a stirred tank bioreactor. The sublytic effects caused by the hydrodynamic stress in combination with insufficient nutrients in the bioreactor may cause cell damage.

引用

页码：1129 / 1133

页数：5

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