Lack of replicative senescence in normal rodent glia

被引：149

作者：

Mathon, NF ^{[1
]}

Malcolm, DS ^{[1
]}

Harrisingh, MC ^{[1
]}

Cheng, LL ^{[1
]}

Lloyd, AC ^{[1
]}

机构：

[1] UCL, MRC, Mol Cell Biol Lab, London WC1E 6BT, England

来源：

SCIENCE | 2001年 / 291卷 / 5505期

关键词：

D O I：

10.1126/science.1056782

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Replicative senescence is thought to be an intrinsic mechanism for Limiting the proliferative Life-span of normal somatic cells. We show here that rat Schwann cells can be expanded indefinitely in culture while maintaining checkpoints normally Lost during the immortalization process. These findings demonstrate that senescence is not an inevitable consequence of extended proliferation in culture.

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页码：872 / 875

页数：4

相关论文

共 14 条

[1] Extension of life-span by introduction of telomerase into normal human cells [J].

Bodnar, AG ;

Ouellette, M ;

Frolkis, M ;

Holt, SE ;

Chiu, CP ;

Morin, GB ;

Harley, CB ;

Shay, JW ;

Lichtsteiner, S ;

Wright, WE .

SCIENCE, 1998, 279 (5349) :349-352

[2] STUDIES ON CULTURED RAT SCHWANN-CELLS .1. ESTABLISHMENT OF PURIFIED POPULATIONS FROM CULTURES OF PERIPHERAL-NERVE [J].

BROCKES, JP ;

FIELDS, KL ;

RAFF, MC .

BRAIN RESEARCH, 1979, 165 (01) :105-118

[3] The biology of replicative senescence [J].

Campisi, J .

EUROPEAN JOURNAL OF CANCER, 1997, 33 (05) :703-709

[4] CALCITONIN-GENE-RELATED PEPTIDE PROMOTES SCHWANN-CELL PROLIFERATION [J].

CHENG, L ;

KHAN, M ;

MUDGE, AW .

JOURNAL OF CELL BIOLOGY, 1995, 129 (03) :789-796

[5] Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19(ARF) [J].

Kamijo, T ;

Zindy, F ;

Roussel, MF ;

Quelle, DE ;

Downing, JR ;

Ashmun, RA ;

Grosveld, G ;

Sherr, CJ .

CELL, 1997, 91 (05) :649-659

[6] SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER [J].

KIM, NW ;

PIATYSZEK, MA ;

PROWSE, KR ;

HARLEY, CB ;

WEST, MD ;

HO, PLC ;

COVIELLO, GM ;

WRIGHT, WE ;

WEINRICH, SL ;

SHAY, JW .

SCIENCE, 1994, 266 (5193) :2011-2015

[7] Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells [J].

Kiyono, T ;

Foster, SA ;

Koop, JI ;

McDougall, JK ;

Galloway, DA ;

Klingelhutz, AJ .

NATURE, 1998, 396 (6706) :84-88

[8] Cooperating oncogenes converge to regulate cyclin/cdk complexes [J].

Lloyd, AC ;

Obermuller, F ;

Staddon, S ;

Barth, CF ;

McMahon, M ;

Land, H .

GENES & DEVELOPMENT, 1997, 11 (05) :663-677

[9] Ras versus cyclin-dependent kinase inhibitors [J].

Lloyd, AC .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1998, 8 (01) :43-48

[10]

MATHON NF, UNPUB