Diverse rhizobia associated with Sophora alopecuroides grown in different regions of Loess Plateau in China

被引:51
作者
Zhao, Longfei [1 ]
Deng, Zhenshan [1 ]
Yang, Wenquan [1 ]
Cao, Ying [1 ]
Wang, Entao [2 ]
Wei, Gehong [1 ]
机构
[1] NW A&F Univ, Coll Life Sci, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China
[2] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Mexico City 11340, DF, Mexico
基金
美国国家科学基金会; 对外科技合作项目(国际科技项目);
关键词
Rhizobia; Sophora alopecuroides; Ecological region; Genetic diversity; Phylogeny; LEGUMINOSARUM BIOVAR VICIAE; SP-NOV; GENETIC DIVERSITY; PHASEOLUS-VULGARIS; PHYLOGENETIC RELATIONSHIP; SEQUENCE-ANALYSIS; NODULATION GENES; ROOT-NODULES; WILD; XINJIANG;
D O I
10.1016/j.syapm.2010.08.004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A total of seventy-five symbiotic bacterial strains isolated from root nodules of wild Sophora alopecuroides grown in different regions of China's Loess Plateau were characterized. Based on the combined RFLP patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including Mesorhizobium alhagi and M. gobiense as the main groups, as well as Agrobacterium tumefaciens, M. amorphae, Phyllobacterium trifolii, Rhizobium giardinii, R. indigoferae, Sinorhizobium fredii and S. meliloti as the minor groups according to the 16S rRNA and recA gene analyses. Five and three lineages of nodA and nifH were found, respectively, in these strains, implying that the symbiotic genes of the S. alopecuroides rhizobia had different origins or had divergently evolved. Results of correspondence analysis showed that there was a correlation between rhizobial genotypes and the geographic origins. Possible lateral transfer of the recA and 16S rRNA genes between the P. trifolii and A. tumefaciens strains, and that of symbiotic genes (nodA, nifH) between different genera, was shown by discrepancies of the phylogenetic relationships of the four gene loci. These results revealed diverse rhizobia associated with wild S. alopecuroides grown in different regions of China's Loess Plateau, and demonstrated for the first time the existence of symbiotic A. tumefaciens strains in root nodules of S. alopecuroides. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码:468 / 477
页数:10
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