Reverse transcriptase-PCR for the detection of Astrovirus in children with nosocomial acute diarrhoea in Naples, Italy

Objective. - This study was made to determine the incidence of nosocomial viral gastroenteritis in all children aged 0-4 years, admitted in the Pediatric Hospital over a 3-year period. Methods. - Astrovirus was detected by reverse transcriptase-PM routine diagnostic tests for Rotavirus, Adenovirus, and common bacterial pathogens were carried out on all samples. Results. - Of the 460 children with nosocomial diarrhea, 23 harbored Astrovirus (5%). Most cases occurred during the coldest months of the year. Children under I year of age were the most susceptible population. Conclusion. - The collected data confirms the importance of viral etiology in nosocomial gastroenteritis. The reported rate of detection stresses the importance of Astrovirus in pediatric diarrhea. The authors recommend screening for this virus on a routine basis. (c) 2005 Elsevier SAS. All rights reserved.