Modification of dengue virus strains by passage in primary dog kidney cells: Preparation of candidate vaccines and immunization of monkeys

被引：51

作者：

Eckels, KH ^{[1
]}

Dubois, DR

Putnak, R

Vaughn, DW

Innis, BL

Henchal, EA

Hoke, CH

机构：

[1] Walter Reed Army Inst Res, Div Communicable Dis & Immunol, Silver Spring, MD 20910 USA

[2] USA, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2003年 / 69卷 / 06期

关键词：

D O I：

10.4269/ajtmh.2003.69.12

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Dengue (DENV) virus strains for each of the four DENY serotypes were modified by passage in primary dog kidney (PDK) cell cultures with final manufacture of vaccine lots in fetal rhesus monkey diploid cell cultures. "Strain sets" consisting of serially-passaged DENV were inoculated in rhesus monkeys along with unmodified parent viruses for each strain. Vaccine candidates were compared with unmodified parent viruses by measuring viremia and immune responses. All except one DENV-1 strain demonstrated reduced infection in monkeys after PDK cell passage. A DENV-3 strain lost all monkey infectivity after PDK cell passage. Twelve vaccine candidates were selected for Phase I human trials through this selection process.

引用

页码：12 / 16

页数：5