Effective removal of albumin from serum

被引：83

作者：

Colantonio, DA

Dunkinson, C

Bovenkamp, DE

Van Eyk, JE

机构：

[1] Queens Univ, Dept Physiol, Kingston, ON K7L 3N6, Canada

[2] Queens Univ, Dept Biochem, Kingston, ON K7L 3N6, Canada

[3] Johns Hopkins Univ, Sch Med, Dept Cardiol, Baltimore, MD USA

来源：

PROTEOMICS | 2005年 / 5卷 / 15期

关键词：

albumin; 2-DE; enrichment; serum proteome;

D O I：

10.1002/pmic.200401235

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein constituents of serum can range from grams to picograms per liter, making it tech- Received: July 16, 2004 nically difficult to achieve in-depth proteomic analysis. Removal of highly abundant proteins, Revised: December 29, 2004 such as albumin, coupled to powerful protein separation methods is required for increased Accepted: January 5, 2005 14 sample load, thus facilitating detection and identification of low-abundant proteins. We report here a chemical-based extraction method for the effective and specific removal of albumin from serum.

引用

页码：3831 / 3835

页数：5

共 14 条

[1] The human plasma proteome - History, character, and diagnostic prospects [J].