Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit

被引：352

作者：

Jaakola, L ^{[1
]}

Pirttilä, AM ^{[1
]}

Halonen, M ^{[1
]}

Hohtola, A ^{[1
]}

机构：

[1] Univ Oulu, Dept Biol, FIN-90014 Oulu, Finland

来源：

MOLECULAR BIOTECHNOLOGY | 2001年 / 19卷 / 02期

关键词：

RNA isolation; CTAB; fruit tissue; bilberry; Vaccinium myrtillus;

D O I：

10.1385/MB:19:2:201

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta -mercaptoethanol. in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.

引用

页码：201 / 203

页数：3

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